Although BAY-I treatment had similar effects on tumor-associated CD4+ T cell and Treg cells in the Pten-null;Cd8-KO mice (Supplementary Fig. 7B), the increased intratumoral CD8+ and GZMb+ cells seen in the Pten-null mice upon BAY-I treatment was nearly completely abolished in Pten-null;Cd8-KO mice (Fig. 5C). The gene discussed is CD8A; the disease is neoplasm.