Importantly, the effects of BAY1082439 on inhibition of cell proliferation and PI3K, IFNα and IFNγ pathways could be observed 24–48 h after a bullet dose on the Pten-null prostate cancer model in vivo (Fig. 1A; Supplementary Fig. 2C and E; Supplementary Data 1) without influence AR expression levels (Supplementary Fig. 2D). This evidence concerns the gene PTEN and prostate cancer.