Using the aforementioned probe design pipeline, we designed 80 probes (two pairs of 40 probes) for the transcript of each gene: BRCA1, BRCA2, CENPF, CKAP5, POLR2A, KI67, MTOR, NCOA1, NCOA2, and NCOA3. These genes were chosen due to their housekeeping status or involvement in tumorigenesis in colorectal cancer. The gene discussed is POLR2A; the disease is colorectal cancer.