PIGR and neoplasm: Using BE cells as the starting point consistent with a previous prostate scRNA-seq study9, we plotted the diffusion pseudotime trajectory and observed that tumor cells and LE cells (KLK3+ ) were later than club cells (PIGR+ , LTF+ , and PSCA+ ) and KRT5+ BE cells (Supplementary Fig. 5a, b), and that there was no significant difference in the computed pseudotime between BE and club cells (Supplementary Fig. 5c).