Using BE cells as the starting point consistent with a previous prostate scRNA-seq study9, we plotted the diffusion pseudotime trajectory and observed that tumor cells and LE cells (KLK3+ ) were later than club cells (PIGR+ , LTF+ , and PSCA+ ) and KRT5+ BE cells (Supplementary Fig. 5a, b), and that there was no significant difference in the computed pseudotime between BE and club cells (Supplementary Fig. 5c). This evidence concerns the gene LTF and neoplasm.