In addition, RNA-binding protein immunoprecipitation assay revealed higher miR-124-3p.1 expression in the AKT2 and SIRT1 groups, thus confirming that miR-124-3p.1 binds directly to AKT2 and SIRT1 in HCC cell lines (Fig. 3C). The gene discussed is SIRT1; the disease is hepatocellular carcinoma.