NTMT1 and retinoblastoma: Next, the enrichment of H3K4me3 in the CENPA promoter region was measured by chromatin immunoprecipitation (ChIP) assay, retinoblastoma cells were transfected with oe-NC or oe-NRMT, and results demonstrated that the expression of H3K4me3 in CENPA promoter region increased remarkably in the cells transfected with oe-NRMT (Fig. 2G), indicating that CENPA could be regulated by NRMT and H3K4me3.