The mRNA and protein expression levels of RSL1D1 were studied in 6 CRC cell lines (SW480, SW620, LoVo, DLD1, HCT116, and RKO), 1 intestinal epithelial cell line (NCM460) and paired CRC tissues with normal counterparts using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot (WB) assays. This evidence concerns the gene RSL1D1 and colorectal carcinoma.