To further investigate the function of RSL1D1 in CRC, we overexpressed RSL1D1 in DLD1 and HCT116 cells by lentivirus transfection (DLD1-Vector/DLD1-RSL1D1, HCT116-Vector/HCT116-RSL1D1). The gene discussed is RSL1D1; the disease is colorectal carcinoma.