To establish the functional role of the interaction between RSL1D1 and RAN in the autophagic program and biological behavior of CRC cells, we used siRNAs targeting RAN to knockdown RAN expression in CRC cells after RSL1D1 overexpression and observed the effects of RAN knockdown on autophagy, proliferation, and invasion. This evidence concerns the gene RSL1D1 and colorectal carcinoma.