Cells were cultured under starvation in Earle’s balanced salt solution (EBSS) for different intervals, and we found that RSL1D1 knockdown aggravated the starvation-initiated autophagic program, while RSL1D1 overexpression attenuated the starvation-initiated autophagic program of CRC cells (Fig. 4A). The gene discussed is RSL1D1; the disease is colorectal carcinoma.