While the pathogenesis of Crohn’s disease is multifactorial and the link to gut permeability is still not well understood, a study in 2019 using three-dimensional tissue culture models demonstrated that epithelial barrier dysfunction may be caused by Tumour Necrosis Factor (TNF)-α induced tight junction modulation and involvement of the c-Jun N-terminal protein kinase mitogen-activated protein kinases (JNK MAPK) signalling pathway [64]. Here, MAPK8 is linked to Crohn disease.