In this sense, the present work aimed to assess the genetic profile of key players associated with the TLR pathway, i.e., TIR domain-containing adaptor protein (TIRAP), MyD88, IRAK1, TNF receptor-associated factor 6 (TRAF6), NFκB inhibitor alpha (IκBα) and NFκB in a stimulated model of AD and after treatment with osthole, in order to evaluate the changes in gene expression signature and secretion of CKs and ChKs. This evidence concerns the gene TIRAP and Alzheimer disease.