Reticulocytes from JAK2V617F PV patients showed increased levels of multiple mitochondria, ribosomal, and ER residing proteins, such as voltage-dependent anion-selective channel protein 3 (Vdac3), 40S/60S ribosomal proteins, and protein disulfide-isomerase (Pdi), respectively, which is consistent with our imaging and flow cytometry data. Here, VDAC3 is linked to acquired polycythemia vera.