Co-culture of CAR T cells with IL-10R+ AML cell lines resulted in CAR T cell activation as shown by upregulation of CD69 and CD25, degranulation by enhanced expression of CD107a, secretion of Granzyme B (GrB), IFN-γ, and TNF-α, and elimination of AML cells in culture. Here, CD69 is linked to acute myeloid leukemia.