This study demonstrated that MLB supplementation attenuated obesity-associated skeletal muscle atrophy in HFD mice through the regulation of the PI3K/Akt/FoxO1 and TNF-α/NF-κB signaling pathways, leading to the inhibition of muscle-specific ubiquitin E3 ligase expression. This evidence concerns the gene AKT1 and obesity due to melanocortin 4 receptor deficiency.