However, the anti-obesity effect of L. plantarum dfa1 was more profound than E. faecium dfa1 as indicated by body weight, blood cholesterol, some regional fat deposition (mesentery, peri-renal, subcutaneous fat, and liver), oxidative stress in the liver, serum pro-inflammatory cytokines (TNF-α and IL-6), and gut leakage (FITC-dextran assay) (Figure 1A–L or Figure 2A–K) without the difference in fecal abundance between both strains of probiotics (Figure 2L). The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.