The mechanism of action was further explored by detecting NF-κB pathway related proteins, and the results confirmed that the three Tieguanyin extracts can down-regulate inflammatory factors such as NF-κB p65, TNF-α, IL-1β, IL-6, COX-2, iNOS, etc., thereby alleviating the symptoms of AD of APP/PS1 mice. Here, TNF is linked to Alzheimer disease.