Figure 3D shows that the β-Gal levels were significantly higher in the SG1 (** p < 0.01) and SG2 (*** p < 0.001) clones than in the EV clones. To confirm the changes in the β-Gal levels, we obtained images of β-Gal-stained cells. A greater number of SA-β-Gal positive cells were observed in the SG1.4 and SG2.7 clones when compared with the OVCAR3 cells or EV clones (Figure 3E). Figure 3F shows the quantification of the number of SA-β-Gal-positive cells, confirming our observation that reduced levels of RBPMS increased the SA-β-Gal in the ovarian cancer cells. The gene discussed is RBPMS; the disease is ovarian carcinoma.