EEF1D and hepatocellular carcinoma: Since the expression of MAT1A and GNMT in HepG2 cells appeared to impede HCC cell proliferation, we further investigated the correlations between the cell doubling time and the expression levels of MAT1A, MAT2A, GNMT, as well as selected REACTOME gene EEF1D and EIF3B by quantitative real-time PCR using designated primers (Figure 4C).