Moreover, real-time RT-PCR showed that the levels of miR-96, miR-527, and miR-2116, which target and silence PYY, GLP-1, and NTS, respectively, in IH-treated cells were not decreased by IH, indicating no involvement of microRNA-mediated posttranscriptional regulation. The gene discussed is GCG; the disease is isolated hemihyperplasia.