Here, to define our ability to map the various anti-HCMV cellular responses post-infection, we used a combination of three pHLA tetramer complexes (HLA-EVMAPRTLIL, HLA-EVMAPRSLLL, HLA-A2NLVPMVATV) and antibodies for 18 surface markers (CD3, CD4, CD8, CD16, CD19, CD45RA, CD56, CD57, CD158, NKG2A, NKG2C, CCR7, TCRγδ, TCRγδ2, CX3CR1, KLRG1, 2B4, and PD-1) in a 20-color multiparameter flow cytometry analysis. Here, CD8A is linked to infection.