CD8A and cytomegalovirus infection: The present study presents the set up and validation of a method based on the use of a set of HLA-Apeptide and HLA-Epeptide complexes in combination with antibodies for 20 cell markers for the concomitant detection, quantification, and immunophenotyping of non-conventional anti-HCMV HLA-EUL40 CD8 T cells, conventional HLA-A*0201pp65 and other immune responses including NK and γδT cell subsets regulated upon HCMV infection.