To characterize the function of CA2 in GBM cells by gain-of-function analyses, stable cell clones of U87 and U251 GBM cell lines were generated by transfection with either a control vector (U87_Ctrl and U251_Ctrl) or with a CA2 plasmid (U87_CA2 and U251_CA2). This evidence concerns the gene CA2 and glioblastoma.