After subcutaneous injection of pcDNA3.1(+) ZKSCAN1 MCS Exon Vector and pcDNA3.1(+) ZKSCAN1 MCS Exon Vector with hsa_circ_0000190 gene using nanoparticle transfection technology, the changes in tumor size and weight of mice were measured twice a week (Figure 3A). The gene discussed is ZKSCAN1; the disease is neoplasm.