The results showed that the H-XRT + α-TIGIT + α-PD1 group was capable of increasing the percentages of total CD4+ T-cells (Figure 4A), CD4+ Foxp3+ Tregs (Figure 4B), as well as total CD8+ T-cells (Figure 4C) at the unirradiated secondary tumor site compared to the Ctrl group (p = 0.05, p = 0.0251, p = 0.0111, respectively). This evidence concerns the gene FOXP3 and neoplasm.