In our current work, we use the single-cell mass cytometry adenocarcinoma panel to investigate (i) the intra- and inter-cell-line heterogeneity of three human adenocarcinoma cell lines, A549, H1975, and H1650; (ii) adenocarcinoma cell line heterogeneity by mimicking a hypoxic environment; (iii) ITH and TMEM45A expression in human primary lung adenocarcinoma versus corresponding healthy human lung tissue used as a control. This evidence concerns the gene TMEM45A and lung adenocarcinoma.