Our data showed that A549, H1975, and H1650 NSCLC adenocarcinoma cells bear intrinsic single-cell heterogeneity due to the expression patterns of the 13 investigated markers GLUT1, MCT4, CA9, TMEM45A, CD66, CD274, CD24, CD326, pan-keratin, TRA-1-60, Gal-3, Gal-1, and EGFR, which could be used to determine intra- and inter-cell-line heterogeneity. This evidence concerns the gene EPCAM and adenocarcinoma.