The clonal heterogeneity of A549, H1975, and H1650 NSCLC cells was determined by the quantitation of the percentages of single cells positive for GLUT1, MCT4, CA9, TMEM45A, CD66, CD274, CD24, CD326, pan-keratin, TRA-1-60, Gal-3, Gal-1, and EGFR. This evidence concerns the gene LGALS3 and non-small cell lung carcinoma.