The clonal heterogeneity of A549, H1975, and H1650 NSCLC cells was determined by the quantitation of the percentages of single cells positive for GLUT1, MCT4, CA9, TMEM45A, CD66, CD274, CD24, CD326, pan-keratin, TRA-1-60, Gal-3, Gal-1, and EGFR. The gene discussed is EPCAM; the disease is non-small cell lung carcinoma.