After completion of treatment with daily IT CpG injection for 5 days with intraperitoneal injections (IP) of anti-OX40/CD134 and anti-CTLA4/CD152 antibodies on days 1 and 5, total splenocytes were co-cultured in the presence of irradiated A20 tumor cells plus ant-CD28 MoAb overnight. The gene discussed is CTLA4; the disease is neoplasm.