Methylation of the INA, NHLH2, and THBS4 loci was also measured in a subset of 136–142 primary tumor tissues free of lymph node or distant metastasis and a total of 202 cancer metastatic tissues isolated from 100 renal cell cancer patients suffering from metachronous metastatic disease (Figure 4b, Table 4). This evidence concerns the gene NHLH2 and renal cell adenocarcinoma.