First, we expressed HA-tagged RISC components (Ago2, TNRC6A TNRC6B) with or without BGLF2-FLAG in HONE-1 (EBV-negative nasopharyngeal carcinoma) cells and stained the cells for HA and FLAG (Figs 2 and 3). The gene discussed is TNRC6B; the disease is nasopharyngeal carcinoma.