Additionally, as CSC generation from cancer non-stem cells can be promoted by a number of transcription factors including TWIST, SNAIL, ZEB1, and ZEB2, the transcription factors that regulate epithelial-mesenchymal transition (EMT), CSC generation is accompanied by EMT [41, 42], which can provide epithelium-derived cancer cells with mesenchymal phenotypes including a motile capacity to exit from the primary sites [43]. This evidence concerns the gene TWIST1 and cancer.