MAP1LC3A and viral infectious disease: To assess whether autophagy was impacted by viral infection, we monitored autophagic flux in cardiomyocytes after SARS-CoV-2 infection using adenovirus expressing a tandem LC3 (light chain 3)-GFP (green fluorescent protein)-RFP (red fluorescent protein) reporter that enables the detection of the autophagic flux process (Figure 5D).