And to examine the PAK4 impact on the occurrence and development of osteosarcoma and osteoblast, we analyzed the online database of OS high-throughput gene chip data through the Kaplan-Meier (http://kmplot.com/analysis/) and found significantly lower overall survival of patients in the PAK4 mRNA high transcript level group compared with the PAK4 low level group (Figure 1(b)). The gene discussed is PAK4; the disease is osteosarcoma.