In order to further verify that miR-10b-5p affects HCC cells by regulating EphA2, we carried out a rescue experiment, finding that pCMV-EphA2 could reverse the downregulation of EphA2 and influences on biological functions caused by overexpression of miR-10b-5p, and Si-EphA2 could reverse the upregulation of EphA2 and influences on cells caused by inhibition of miR-10b-5p, which further proved that miR-10b-5p exerted its effect on HCC cells by regulating EphA2. The gene discussed is EPHA2; the disease is hepatocellular carcinoma.