We first examined the distribution of prognostic MSG expression among five immune subtypes reported by a recent study (Thorsson et al., 2018) and found that there was a significant difference in GSS, GMPPA, SGPP2, and PIK3CA among the five immune subtypes (Supplementary Figure S10), suggesting that most prognostic MSGs are closely associated with the tumor immune microenvironment. This evidence concerns the gene PIK3CA and neoplasm.