Using the multiplex assays, we found that infection of 3-D cervical cell models with M. mulieris significantly upregulated expression of IL-6 (p<0.0001), IL-8 (p<0.0001), MCP-1 (p<0.01) and TNF-α (p<0.01) whereas infection with Eggerthella sp. This evidence concerns the gene TNF and infection.