Furthermore, the mRNA expression levels of the nine TRIM family members (TRIM3, TRIM5, MID1, TRIM21, TRIM27, TRIM32, TRIM44, TRIM47, and TRIM72) were detected in human HCC cell lines (HepG2, Huh7, MHCC97H, Hep3B, and SK-Hep-1) and normal human hepatocyte cell line MIHA by qRT-PCR assays (Figure 4A). The gene discussed is MID1; the disease is hepatocellular carcinoma.