demonstrated that intravenous injection of JX-594, an engineered vaccine virus with TK deletion and overexpression of human granulocyte-monocyte colony-stimulating factor (hGM-CSF), led to replication of the virus in endothelial cells of the nearby tumor and disrupted tumor blood flow, which ultimately ended in intensive tumor necrosis within 5 days. This evidence concerns the gene TKT and neoplasm.