Consequentially, DNAM+ expression levels were lower in the intratumoral NK cells, CD4+, and CD8+ T cells, suggesting a less functional phenotype of T and NK cells in the tumor microenvironment, as DNAM expression is related to better IFN-γ production.33 Also, CX3CR1+ expression levels in the CD8+ T cells and NK cells were higher in the PB samples compared to the tumor cells. This evidence concerns the gene CD4 and neoplasm.