There was a significant difference among grade 1, grade 2, and grade 3 (P < 0.05; Figure 5(a)), whereas CYP39A1 mRNA expression was not correlated with cancer stage, age, nodal metastasis, gender, or histological type of HCC (P > 0.05; Figures 5(b)–5(f)). This evidence concerns the gene CYP39A1 and hepatocellular carcinoma.