Gli1 was silenced in HCT116 cells with or without LATS1 depletion to see if it was involved in LATS1 regulation of CRC cell proliferation and migration and it was observed that LATS1 knockdown promoted the migration and proliferation of HCT116 cells, but the enhanced proliferation and migration ability was greatly impaired when Gli1 was depleted, indicating the dependence of anti-carcinogenic roles of LATS1 on Gli1. This evidence concerns the gene GLI1 and colorectal carcinoma.