TP53(T-low: 56.5%, T-high: 79.4%; p = 0.006) and BRAF (T-low: 0%, T-high: 11.9%; p = 0.012), which are known frequently mutated genes in colorectal cancer, showed more frequent mutations in the patient group of T3 and T4 stages (T-high). The gene discussed is TP53; the disease is colorectal cancer.