All the glioma cell line featured with a significantly higher CPNE3 mRNA (Fig. 2A) and protein (Fig. 2B) expression when compared with HEB cell line, and the glioma cell lines with the highest (U251) and the lowest (T98G) expression were selected for the CPNE3 knock-down and overexpression study. The gene discussed is CPNE3; the disease is glioma.