However, PIWIL1 positive expression was significantly associated with deeper tumor invasion (n = 5, OR = 2.26, 95% CI: 1.09–4.70, p=0.03), higher clinical stage (n = 6, OR = 1.53, 95% CI: 1.09–2.14, p=0.01), and more lymph node metastasis (n = 4, OR = 1.90, 95% CI: 1.25–2.88, p=0.003). This evidence concerns the gene PIWIL1 and metastatic malignant neoplasm in the lymph nodes.