The results showed that the expression of TYMS was negatively correlated with tumor mutational burden (Figure 5C) and microsatellite instability (Figure 5D), and positively correlated with the expression of four methyltransferases (Figure 5E, DNMT1: red, DNMT2: blue, Dnmt3A: green and DNMT3B: purple). The gene discussed is TYMS; the disease is neoplasm.