After co-culture with TAK-1 cells, the inhibition rate of CD34+CD59- cell colony formation in 5 PNH patients was significantly lower than that of CD34+CD59+ cells, suggesting that WT1 peptide-specific and HLA-restricted cytotoxic T cells may play an important role in immune escape of PNH, which may be related to interferon-γ (43). This evidence concerns the gene CD34 and paroxysmal nocturnal hemoglobinuria.