To verify the results of our data analysis, we extracted total RNA from different tumour cell lines (HCT116, SW480, HT29, LOVO, RKO, DLD-1) and the normal epithelial colon cell line NCM460 and measured the mRNA expression levels of TIMP1, PLCG2, BDNF and IL13. The gene discussed is BDNF; the disease is neoplasm.