Hydrodynamic injection of miR-15a/16-1 (containing the miR-15a and miR-16-1) can prevent HCC in both protein kinase B (AKT)/Ras and c-Myc mice with overexpression of activated forms of AKT and NRas oncogenes (AKT/Ras) or c-Myc, via suppressing Tregs function to increase the effect of cytotoxic T cells (90). The gene discussed is AKT1; the disease is hepatocellular carcinoma.