One of the limitations of our study is that, although we demonstrated that TRPV4 mediated CSE-induced pyroptosis via the Ca2+/NLRP3/Caspase-1/GSDMD axis in vitro experiments, the significance of pyroptosis and this signaling pathway in COPD pathogenesis still needs further investigation in mouse models and human COPD. The gene discussed is CASP1; the disease is chronic obstructive pulmonary disease.