In addition, mRNA and protein levels of PLCβ1 are increased in ischemia (Asemu et al., 2003) and in stroke-prone SHRs as young as 8–10 months there is increased sarcolemmal protein kinase C (PKC) activity (Kawaguchi et al., 1993), suggesting increased PLCβ1 activity. This evidence concerns the gene PRRT2 and ischemia.