An initial snRNA-seq analysis of the brain tissues from three patients with Mendelian or sporadic AD showed that it is possible to identify different cell types from frozen brains of patients with different forms of AD and discovered five differentially expressed genes (EEF1A1, GLULL, KIAA1217, LDLRAD3, and SPP1) that are consistently associated with microglia in all three samples (Del-Aguila et al., 2019; Table 2). This evidence concerns the gene SPP1 and Alzheimer disease.