In summary, these results imply that C/EBPβ participates in PARP1-initiated cardiac hypertrophy, PARP1 positively regulate C/EBPβ protein level and DNA binding activity without affecting its mRNA level, suggesting that this regulation is not at the transcriptional level, but is probably attributed to the stability or degradation of C/EBPβ protein. Here, CEBPB is linked to cardiac hypertrophy.