CASP9 and neoplasm: Consistent with previous in vitro experiments, Ad-vp3 and Ad-vt treatment increased the levels of cleaved-PARP, cleaved-GSDME, cleaved-caspase-3, cleaved-caspase-9, and up-regulated the expression of bax and cytochrome C. In summary, apoptin can inhibit tumor growth and induce pyroptosis in vivo.