In the present study, we found that RocA damaged mitochondrial DNA (mtDNA) and promoted the cytoplasmic release of mtDNA, leading to the activation of cGAS (cyclic GMP-AMP synthase)-STING (stimulator of interferon genes) signaling pathway and increased NK cell infiltration in NSCLC. The gene discussed is CGAS; the disease is non-small cell lung carcinoma.