To confirm the in vivo interactions between Puf-A and p53, we employed a previously established lung cancer model using CCSP-rtTA/TetO-Cre/LSL-KrasG12D/p53+/+ mice [27], which developed lung adenoma, followed by ADC at 6 and 12 weeks, respectively, after KrasG12D induction (Fig. 4A). Here, TP53 is linked to lung cancer.