To understand the interplay between Puf-A and p53 in cancer cells, we suppressed Puf-A expression in p53+/+-A549, p53+/+-H460, p53+/+-HCT116, p53−/−-HCT116, p53−/−-H1299, and p53R248W-CL1-5 cells by infection with shPuf-A-1 or shPuf-A-2 lentivirus. The gene discussed is TP53; the disease is cancer.