We developed the live cell CELsignia clinical test for quantifying dysfunctional signaling and then applied basic scientific process to pre-clinically verify and identify an estimated cutoff of signaling amount and the prevalence in a statistically significant population of 79 histopathologically HER2-negative breast cancer patients with concomitant c-Met and HER family-driven signaling activity. The gene discussed is ERBB2; the disease is breast carcinoma.